Quantify HiBiT-Tagged Proteins Expressed on the Cell Surface
- Specific, live-cell detection of extracellular expressed or secreted proteins
- Simple add-mix-read assay format—no antibodies required
- Quantitate over 7 logs of linear dynamic range
Simple Bioluminescent Detection of Cell-Surface Protein Expression
The Nano-Glo® HiBiT Extracellular Detection System quantitates cell surface or secreted protein expression in minutes using a simple add-mix-read assay format.
Using the nonlytic Nano-Glo® HiBiT Extracellular Detection Reagent, any proteins that are tagged with the 11 amino acid HiBiT peptide and expressed outside of the cell can be specifically quantitated. The detection reagent contains the complementary polypeptide LgBiT, which spontaneously interacts with the HiBiT tag to reconstitute the bright, luminescent NanoBiT® enzyme.
Luminescence is proportional to the amount of HiBiT-tagged protein present outside of the cell over seven orders of magnitude.