The NanoBRET assay is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc Luciferase as the BRET energy donor and HaloTag protein labeled with the HaloTag NanoBRET 618 fluorescent Ligand as the energy acceptor to measure the interaction of two binding partners in live cells. The NanoBRET Nano-Glo Detection System provides the NanoBRET Nano-Glo Substrate used by NanoLuc Luciferase to generate the donor signal and the HaloTag NanoBRET 618 Ligand for the fluorescent energy acceptor. The HaloTag NanoBRET 618 Ligand is added directly to the cells during plating, and the NanoBRET Nano-Glo Substrate is added to the sample just prior to measuring donor and acceptor emission. For more details on using the NanoBRET assay for protein:protein interaction studies visit the NanoBRET Technology for Protein Interactions page.|
Understand Real Biology: Live-cell reagents allow you to detect protein:protein interactions in real time using full-length proteins or fragments.
Monitor Changes: Reversible assay technology allows you to study both induction and inhibition of protein interactions.
See Improved Assay Performance: Bright, blue-shifted donor signal and red-shifted acceptor create optimal spectral overlap, increased signal and lower background compared to conventional BRET assays.
Scale Your Assays: Assays can be performed in 96- or 384-well formats with low variability and high reproducibility.
Enjoy Convenience: The NanoBRET(TM); Nano-Glo(R), Detection System is compatible with a diverse set of pre-built or custom NanoBRET(TM); PPI Assays.