Wizard Plus Megapreps DNA Purification System provides a simple and rapid method for large-scale purifications of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high-copy-number plasmid DNA (200-20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511).|
Fast: Rapid batch binding and column washing method used for DNA isolationSafe: Eliminates the need for cesium chloride:ethidium bromide gradient centrifugation and does not require organic extractions.Reliable: Yields plasmid DNA of comparable quantity and quality to cesium chloride:ethidium bromide gradient techniques that are much more time- and labor-intensive.Yield: Each megaprep produces more than1mg of DNA from 1,000ml of bacterial culture when using a high-copy-number plasmid.Quality: DNA is suitable for restriction enzyme digestions, automated fluorescent DNA sequencing, transformation and subcloning.