Promega

Magnesium Acetate

Varenummer: L4581
Kort informasjon 100µl
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The TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TNT Quick Master Mix. The TNT Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2-2.0ug of circular plasmid DNA containing a T7 or SP6 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TNT Quick Master Mix and incubated in a 50ul reaction volume for 60-90 minutes at 30 degrees C. The expression reaction produces significant quantities of protein for a variety of applications including GST pull-downs and gel shift assays.|
Obtain Data Faster: Functional protein is expressed in only one hour, not days as with cell-based expression systems.Multiple Applications with One System: Use expressed protein for the characterization of protein:protein interaction, protein:nucleic acid interaction, protein modification and more.Consistent, Reliable Results: This mammalian-based system expresses soluble, functional proteins that are post-translationally modified, unlike E. coli-based systems.Fewer Steps: Expressed proteins can be used directly after expression; no requirement for additional purification.Flexible Systems Available: TnT(R) Systems for linear, circular or PCR templates are available.

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Store at -70°C. Do not freeze-thaw the lysate more than two times.|U.S. Pat. Nos. 5,641,641 and 5,650,289. U.S. Pat. No. 5,552,302. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product. Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.

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Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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