The LigaFast Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg(2+) addition prior to use.|
Flexible: Use with 5´, 3´ or blunt-ended DNA inserts.Fast: Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature.Convenient: No requirement to purify ligated DNA prior to heat-shock transformation in E. coli. Ligations conducted at room temperature.Ready-To-Use: No additional buffer modifications required prior to use.Efficient: Ligations performed using the LigaFast™ System are comparable to standard overnight ligations.Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.