Promega

pGEM-3Zf(+) Vector

Varenummer: P2271
Kort informasjon 20 µg
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The pGEM-3Zf(+) and pGEM-3Zf(-) Vectors are derived from the pGEM-3Z Vector and contain the origin of replication of the filamentous phage f1. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites forEcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM-3Zf(+) and -3Zf(-) Vectors are identical except for the orientation of the f1 origin and can be used as standard cloning vectors, as templates for in vitro transcription andfor the production of circular ssDNA.|
Blue/White Screening: Allows the easy identification of recombinant clones.Versatile: These vectors can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

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Ekstra spesifikasjoner
Store vector at -20°C and bacterial strain at -70°C.|

Kontaktperson(er) til dette produktet

Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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