Flowcytometri

The CD66c antigen, also known as non-specific cross-reacting antigen (NCA90), is a granulocyte cell surface GPI-linked protein of 80-100 kDa. It is also called KOR-SA3544.

Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells.

Lactoferrin is a 76 kDa single chain polypeptide with iron-binding property. This protein shows structural similarity to the plasma iron-transport protein transferrin.

The LeukoSure Enumeration Kit is comprised of a set of reagents used to prepare and enumerate white blood cells in leukoreduced red blood cell and platelet products by flow cytometry. The enumeration method depends upon mixing a known volume (100 μL) of LeukoSure Fluorospheres with an identical volume of sample to be tested. After analysis the absolute count for the specimen is calculated. This represents the absolute number of leukocytes in the specimen. The specimen is lysed and permeabilized using the LeukoSure Lyse Reagent to eliminate RBCs and prepare the cells for subsequent addition of the LeukoSure Stain Reagent which contains propidium iodide and RNAse (enzyme used to remove ribonucleic acid, or RNA). In the absence of RNA, propidium iodide binds only to double stranded deoxyribonucleic acid (DNA) so that nucleated cells in the sample emit fluorescence in proportion to their DNA content. The flow cytometer measures the fluorescence from each stained cell as it passes through the laser beam. Since mature platelets and red blood cells do not contain DNA, the stained cells represent the leukocyte component of the blood.

The Lineage-PE Cocktail is comprised of PE-conjugated CD3, CD14, CD19, CD20 and CD56 antibodies.

Myeloperoxydase (MPO) is an heterodimeric glycoprotein of 150 kDa with an α2/β2 structure. The two subunits (α and β) have a molecular weight of 55 and 15 kDa, respectively.

Myeloperoxydase (MPO) is an heterodimeric glycoprotein of 150 kDa with an α2/β2 structure. The two subunits (α and β) have a molecular weight of 55 and 15 kDa, respectively.

IOTest Conjugated Antibodies are: Ready-to-use conjugated monoclonal or polyclonal antibodies for flow cytometric analysis. Available as single or multi-color configurations for direct immunofluorescent staining. Adapted for manual lysing procedures with a wash and for automated lysing procedures on the PrepPlus2 Workstation and the FP 1000 Cell Preparation System.

The NKp80, also known as killer cell lectin-like subfamily F, member 1 (KLRF1) is a type II transmembrane glycoprotein of 80 kDa and 40 kDa under reducing conditions.

The natural killer (NK), T lymphocyte, B lymphocyte antigen (NTB-A) is a 60-kDa transmembrane glycoprotein of the CD2 family expressed on all human resting and activated NK, T and B cells.

OSCAR, an abbreviation of osteoclast-associated receptor, is a new member of the leukocyte receptor complex. Human OSCAR is a cell-surface, monomeric N-glycosylated protein of approximately 45 kDa.

This reagent is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis after staining of leucocytes with fluorescent antibodies. Use of OptiLyse C is limited to samples for analysis using Beckman Coulter flow cytometers and is compatible with so-called « no wash» staining and lysis procedures so long as duly calibrated fluorescent antibodies are used for this purpose.

This reagent is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis after staining of leucocytes with fluorescent antibodies. Use of OptiLyse C is limited to samples for analysis using Beckman Coulter flow cytometers and is compatible with so-called « no wash» staining and lysis procedures so long as duly calibrated fluorescent antibodies are used for this purpose.

This reagent is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis after staining of leucocytes with fluorescent antibodies. Use of OptiLyse C is limited to samples for analysis using Beckman Coulter flow cytometers and is compatible with so-called « no wash» staining and lysis procedures so long as duly calibrated fluorescent antibodies are used for this purpose.

This package contains 15 foil-wrapped packets of PBS Buffer reagent. Each package is brought up to a 500 mL volume with distilled water to yield a 0.01 M potassium phosphate, 0.15 M sodium chloride at a pH of 7.2 ± 0.2.