Beckman Coulter Life Sciences
OptiLyse No-Wash Lysing Solutions 250 tests RUO
This reagent is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis after staining of leucocytes with fluorescent antibodies. Use of OptiLyse C is limited to samples for analysis using Beckman Coulter flow cytometers and is compatible with so-called « no wash» staining and lysis procedures so long as duly calibrated fluorescent antibodies are used for this purpose.
The biological sample containing red blood cells for lysis is incubated in the presence of the OptiLyse C solution, which results in the lysis of red blood cells accompanied by the fixation of leucocytes.
A specific staining of leucocytes is first achieved by incubating the sample with the antibody or antibodies selected. The red cells are then removed by lysis and the leucocytes, which are fixed during this stage, are analyzed by flow cytometry.
Preparations must be stored between 2 and 8°C and rapidly analyzed by flow cytometry without prior washing being necessary.