NextGenPCR™ MPXV Sequencing Library Prep Kit 96RXN

NextGenPCR™ MPXV Sequencing Library Prep Kit 96RXN

MBS is supplying a full library prep kit for MPX sequencing. After an ultrafast PCR, the product is ready for nanopore sequencing.

The NextGenPCR™ MPXV library preparation protocol was tested on 40 samples in close collaboration with Matthijs Welkers and Marcel Jonges from the Dept. of Medical Microbiology & Infection Prevention at Amsterdam UMC (location AMC), The Netherlands.

Using NextGenPCR™ MPXV library preparation up to 20 samples could be multiplexed in a single nanopore flowcell with greatly improved sequencing depth and coverage owing to its PCR tiling based target enrichment. This additionally results in lower costs per sample. The NextGenPCR™ MPXV library preparation method was successfully employed to sequence the first known case of MPXV infection in The Netherlands.

For more details, see also our NextGenPCR™ MPXV Amplification Primer Panel (96 reactions)