Promega

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

M-MLV RT brukt i cDNA-syntese med lange RNA-templat. Mangler RNase H-aktivitet, gunstig for å forberede lange cDNAer og full lengde cDNA-biblioteker.

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H-]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when synthesizing long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.