Promega

GoTaq Hot Start Polymerase

GoTaq Hot Start Polymerase contains the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two minutes.

GoTaq Hot Start Polymerase contains the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94-95 degrees C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 degrees C.