Beckman Coulter Life Sciences

IOTest Beta Mark TCR Vb Repertoire Kit 25 tests RUO

The IOTest Beta Mark is a multi-analysis tool designed for quantitative analysis of the TCR Vβ repertoire of human T lymphocytes by flow cytometric analysis. Using an innovative staining principle (see below), this kit simplifies the TCR Vβ repertoire analysis by reducing the number of tubes to analyze (8 instead of 24) and consequently the time required for obtaining the results. Moreover, this kit makes it possible to study the repertoire on T cell subsets, using antibodies to additional T-cell markers, such as CD3, CD4, and CD8. The kit is composed of 8 vials containing mixtures of conjugated TCR Vβ antibodies corresponding to 24 diffe rent specificities (about 70% coverage of normal human TCR Vβ repertoire).

The test is intended for use on whole blood samples*. The staining protocol includes a one-step procedure with directly FITC- and PE-coupled antibody mixes (test volume of 20μl) and a wash step after staining. A third color T-cell marker (preferentially as PC5-conjugate; not included) is used to gate the specific population. The kit can be used in 3 or in 4 color settings. The test is compatible with different methods of lysis and can be run on Beckman Coulter as well as B-D cytometers.

The IOTest Beta Mark includes a control for fluorescence PMT and compensation settings. Reagent A is used for this purpose without addition of a T-cell marker (to include 25 control tests, vial A contains 50 tests).

* Other blood preparations, such as PBMC (ficolled blood samples), and other body fluids may be processed by the IOTest Beta Mark but the procedure has not been standardized. The IOTest Beta Mark is intended For Research Use Only. Not For Use In Diagnostic Procedures.

Sikkerhetsdatablad

IM3497