Promega

T7 RiboMAX Express RNAi System

The T7 RiboMAX Express RNAi System is an in vitro transcription system designed to produce milligram amounts of RNA suitable for use in RNAi assays.

The T7 RiboMAX Express RNAi System is an in vitro transcription system designed to produce mg amounts of dsRNA in a short amount of time. The dsRNA is free of protein and other contaminants and suitable for use in RNAi in both mammalian and nonmammalian systems. The system can be used to synthesize 21bp siRNAs for use in mammalian systems. siRNAs synthesized in vitro have shown to be as effective as chemically synthesized siRNAs for inducing RNAi in mammalian cells. Also use the system for the synthesis of dsRNA molecules of approximately 200bp or greater, which can be applied to nonmammalian systems. Two complementary RNA strands are synthesized from DNA template (either plasmid or PCR product). The resulting RNA strands are annealed after the transcription reaction to form dsRNA. Remove any remaining ssRNA and DNA template with a nuclease digestion step. Then purify the dsRNA by isopropanol precipitation, and introduce into the organism of choice for RNAi applications.

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