Conventional DNA Digest Markers are created by digesting either lambda DNA, PhiX174 replicative form DNA, or plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. PhiX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 72bp to 1,353bp. Recommended Loading: Load 1microl/lane.