![GoTaq Hot Start Colorless Master Mix](/storage/3238674CA6AFF899417EC01FBADD47BD7B0F7225DB6E3508914B16B9E0D4277C/ae49b9f34d634240b0b022a1a13adebd/840-840-0-png.Png/media/728da9bcbaa843748be6ea774ec4b930/M5132_GoTaq%20Hot%20Start%20Colorless%20Master%20Mix_2.png)
![GoTaq Hot Start Colorless Master Mix](/storage/CADAEAC8A9066B4ECC99B5869B848DB1876AE163761704276609CAAE1FADCB6C/98ea86540da54454a766d47fb2d53026/840-840-0-png.Png/media/74163922a3c64413b89128afa0ef78c7/M5133_GoTaq%20Hot%20Start%20Colorless%20Master%20Mix_2.png)
Promega
GoTaq Hot Start Colorless Master Mix
GoTaq Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq Hot Start Polymerase, magnesium, dNTPs and buffer. Set up reactions in less than a minute at room temperature; just add your template, water and primers. GoTaq Hot Start Polymerase contains the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94-95 degrees C for two minutes, enabling hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 degrees C. GoTaq Hot Start Polymerase exhibits 5' --> 3' exonuclease activity. The master mixes are available in green or colorless formats. The green master mix contains a compound that increases sample density, so samples sink easily into wells of an agarose gel, and two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis.