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Promega
AccessQuick RT-PCR System
The AccessQuick RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. The AccessQuick Master Mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. The AccessQuick RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.