PureYield Plasmid Midiprep System

The PureYield Plasmid Midiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify 100-200ug of plasmid DNA from a 50ml bacteria culture. Purify plasmid DNA in as little as 30 minutes with the vacuum protocol, greatly reducing time spent compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400ug of high-copy-number plasmid from 250ml of E. coli culture. The system incorporates a unique Endotoxin Removal Wash to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Purify without isopropanol precipitation or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).