Promega

PureYield Plasmid Maxiprep System

The PureYield Plasmid Maxiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify up to 1mg of plasmid DNA from a 250ml bacterial culture. Purify plasmid DNA rapidly with the vacuum protocol, greatly reducing time spent compared to silica resin or other membrane-column methods. This system incorporates a unique Endotoxin Removal Wash to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Purify without isopropanol precipitation. Use the Eluator Vacuum Elution Device to elute nucleic acids from PureYield Midiprep or Maxiprep columns. The device base interfaces with a Vacuum Manifold that contains Luer-Lok fittings, such as the Vac-Man Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield Midiprep or Maxiprep columns. The device eliminates the need for a centrifuge with a swinging bucket rotor for nucleic acid purification.