SAM2 Biotin Capture Membrane

The SAM2 Biotin Capture Membrane binds biotinylated molecules based on their affinity for streptavidin. The proprietary process by which the SAM2 Membrane is produced results in a high density of streptavidin on the filter, providing rapid, quantitative substrate binding in the nmol/cm(2) range, depending upon the substrate used. In addition, the membrane is designed to minimize nonspecific binding. The membrane is available as a large, prenumbered, partially cut sheet (approximately 10.5 x 15.0cm). The partially cut membrane allows easy separation into 96 individual squares and is designed for small-scale experiments where high binding capacity is required. The membrane may be analyzed using phosphorimaging analysis, autoradiography or scintillation counting to quantitate results. The membrane also has been used successfully with chemiluminescence detection techniques. The use of fluorescence for detection of captured molecules is not recommended at this time.