PKC iota Kinase Enzyme System

Recombinant full-length human PKC iota was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C iota is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKC iota showed greatest homology to PKC zeta, with 72% identity overall rising to 84% in the catalytic domain. PKC iota has been implicated in Ras signaling and is a critical downstream effector of oncogenic Ras in the colonic epithelium. Transgenic mice expressing constitutively active PKC iota in the colon are highly susceptible to carcinogen-induced colon carcinogenesis. Kinase Enzyme System contains: Kinase: PKC iota, 10ug (Human, recombinant full-length). MW: ~98kDa. Substrate: CREBtide (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acid 109-121). Other: Reaction Buffer, DTT, Lipid solution. PKC iota NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5584/.