Promega

Control Ab, Anti-4-1BB

The 4-1BB Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate 4-1BB.

The 4-1BB Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate 4-1BB. The assay consists of a genetically engineered Jurkat T cell line that expresses human 4-1BB and a luciferase reporter driven by a response element that can respond to 4-1BB ligand/agonist antibody stimulation. The 4-1BB Effector Cells are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. Control Ab, Anti-4-1BB, is a mouse anti-human 4-1BB agonist antibody. It can be used as a positive control in the 4-1BB Bioassay in the presence of FcγRIIb CHO-K1 Cells (Cat.# JA2251, JA2255).