Promega
AXL Kinase Enzyme System
rhAXL (amino acids 473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. AXL is a member of the receptor tyrosine kinase family, which has oncogenic potential and is implicated in human myeloid leukemia. AXL is a member of a complex signaling network that is involved in the control of cell proliferation and differentiation. Overexpression of AXL cDNA in NIH3T3 cells induces neoplastic transformation of these cells with the concomitant appearance of a 140kDa AXL tyrosine-phosphorylated protein. Expression of AXL cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by anti-phosphotyrosine antibodies. Kinase Enzyme System contains: Kinase: AXL, 10ug (Human, recombinant; amino acids 473-end). MW: ~55kDa. Substrate: Axltide (KKSRGDYMTMQIG); derived from the mouse Insulin receptor substrate 1 (amino acid 979-989). Other: Reaction Buffer, DTT. AXL NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/558/.