MARK1 Kinase Enzyme System

Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction. Due to its protein serine/threonine kinase activity, MARK1 is known to mediate phosphorylation of key proteins involved in signal transduction and cell communication. MARK1 phosphorylates microtubule-associated proteins and triggers microtubule disruption (1). Gene mutation studies performed in mice revealed that, after targeted disruption of the MARK1 gene, the mice lacked the ability to drink and displayed hind leg motor dysfunction (2). Kinase Enzyme System contains: Kinase: MARK1, 10ug (Human, recombinant full-length). MW: ~125kDa. Substrate: Chktide (KKKVSRSGLYRSPSMPENLNRPR); derived from human CDC25C protein isoform A (amino acids 205-225). Other: Reaction Buffer, DTT. MARK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4139/.