Proteasome-Glo Caspase-Like Assay

The Proteasome-Glo Cell-Based Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. The 26S proteasome is a 2.5MDa multiprotein complex found in all eukaryotic cells. Adding the Proteasome-Glo Cell-Based Reagent in an add-mix-measure format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction. The three luminogenic substrates used to monitor specific protease activities include: Suc-LLVY-aminoluciferin for chymotrypsin-like, Z-LRR-aminoluciferin for trypsin-like, and Z-nLPnLD-aminoluciferin for caspase-like activity. Each substrate is added to a buffer system optimized for its specific proteasome activity and luciferase activity. The Proteasome-Glo 3-Substrate System and Proteasome-Glo Cell-Based 3-Substrate System are also available.