Beckman Coulter Life Sciences
OptiLyse No-Wash Lysing Solutions 250 tests RUO
OptiLyse B is an erythrolytic reagent for lysing human red blood cells following direct immunofluorescence staining of whole blood for flow cytometry. OptiLyse B is a ready-to-use lysing solution optimized for use on BD Biosciences instruments.
The biological sample containing red blood cells for lysis is incubated in the presence of the OptiLyse C solution, which results in the lysis of red blood cells accompanied by the fixation of leucocytes.
A specific staining of leucocytes is first achieved by incubating the sample with the antibody or antibodies selected. The red cells are then removed by lysis and the leucocytes, which are fixed during this stage, are analyzed by flow cytometry.
Preparations must be stored between 2 and 8°C and rapidly analyzed by flow cytometry without prior washing being necessary.