FastCellular Senescence Detect SPiDER-beta-Gal live/fix.cells

Cellular senescence is a phenomenon by which normal cells cease to divide and enter a state of irreversible growth arrest. It is one of the most fundamental aspects of cellular behavior and generally believed to be associated with tumor suppressive mechanisms and an underlying cause of aging. Senescence-associated Β-galactosidase (SA-Β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Although X-gal is a well-known reagent used to detect SA-Β-gal, X-gal has the following disadvantages: 1) requirement of fixed cells due to poor cell-permeability, 2) low quantitative capability because of the difficulty in visually differentiating stained vs. non-stained cells 3) requirement for long stain times.