Promega

Wizard Plus Minipreps DNA Purification System

A simple, reliable, silica-resin-based method for rapid isolation of plasmid DNA on a miniprep scale. No organic extraction or ethanol precipitation required. Extraction completed in 15 minutes.

The resin-based Wizard Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. When using the standard protocol, the process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Process minipreps individually or in multiples with the Vac-Man (20-sample capacity, Cat.# A7231) or Vac-Man Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. Elute DNA from the Wizard Minicolumn in Nuclease-Free Water (Cat.# P1193). Purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor, such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511). Use the Wizard Minipreps DNA Purification Resin, available with the system or as a standalone product, in conjunction with the Wizard Plus Minipreps DNA Purification System.

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