Promega

HaloTag Oregon Green Ligand

The HaloTag Technology enables covalent protein labeling and immobilization in vivo and in vitro.

The HaloTag Fluorescent Ligands offer a variety of functionalities, including fluorescent labels, affinity tags and solid phase attachment abilities. The ligands form covalent bonds rapidly under general physiological conditions; the bonds are highly specific and essentially irreversible. HaloTag Fluorescent Ligands enable you to add the chloroalkane group, with which HaloTag protein reacts, to any compound or surface with a compatible chemical group. The possible applications are endless. The Alexa Fluor 488 Ligand is impermeable to cell membranes and, therefore, used to label cell surface proteins. The TMR Ligand, Oregon Green Ligand, diAcFAM Ligand and Coumarin Ligand readily cross the cell membrane and, therefore, can be used to label intracellular proteins.