![10X Flexi Enzyme Blend (SgfI & PmeI)](/storage/F7B1155C21DCA7D50F886040F56B38CD128498A6EB792FB152084419CDDD548D/81c8621d51b74b4a85959b54f67efc76/840-840-0-png.Png/media/117180adc63541c193ddc61b998edbd2/R1851_10X%20Flexi%20Enzyme%20Blend%20%28Sgf%20I%20and%20Pme1%29_2.png)
![10X Flexi Enzyme Blend (SgfI & PmeI)](/storage/61DC04772CCD5F9D1A45E2A85BBDCF83F05C674BF92456170A156718B11B448E/cd8d013baedb45e59b6edfda2ec308cc/840-840-0-png.Png/media/f5fa4c4fc52d4dcf848c980d33d01fd1/R1852_10X%20Flexi%20Enzyme%20Blend%20%28Sgf%20I%20and%20Pme1%29_2.png)
Promega
10X Flexi Enzyme Blend (SgfI & PmeI)
The 10X Flexi Enzyme Blend (SgfI and PmeI) is required to screen Flexi Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi Vectors or transfer protein-coding regions between Flexi Vectors. For more information see the Flexi Vector Systems Technical Manual #TM254. Recognition Sites: SgfI recognition site: 5…GCGATtCGC…3 3…CGCsTAGCG…5. PmeI recognition site: 5…GTTTtAAAC…3 3…CAAAsTTTG…5. Storage Buffer: The 10X Flexi Enzyme Blend (SgfI and PmeI) is supplied in 10mM Tris-HCl (pH 7.4 at 25°C), 150mM KCl, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.35mg/ml BSA, 0.05% Thesit and 50% glycerol. 5X Flexi Digest Buffer: The composition of 5X Flexi Digest Buffer is 50mM Tris-HCl (pH 7.9 at 37°C), 50mM MgCl2, 250mM NaCl, 5mM DTT, 0.5mg/ml acetylated BSA.
Denne teksten er oversatt ved hjelp av AI og kan inneholde unøyaktigheter.