Bacterial Strain JM109, Glycerol Stock

Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening. Genotype: endA1, recA1, gyrA96, thi, hsdR17 (r(k)-, m(k)+), relA1, supE44, Delta(lac-proAB), [F' traD36, proAB, lacI(q)ZDeltaM15].