Recombinant human VRK2 (1-375) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. VRK2 (also known as vaccinia related kinase 2) is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. VRK2 is widely expressed in human tissues and highly expressed in actively dividing cells, such as those in testis, leukocytes, fetal liver and carcinomas (1). VRK2 can be used to phosphorylate casein and itself undergo autophosphorylation. VRK2 interacts specifically with Epstein-Barr virus BHRF1, a homologue of Bcl-2, and this interaction is involved in protecting cells from apoptosis (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: VRK2, 10ug (Human, recombinant; amino acids 1-375). MW: ~66kDa. Substrate: Native Casein Protein was purified from bovine milk. Other: Reaction Buffer, DTT, MnCl2. VRK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/7444/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.|
Profile More Compounds In-House: ADP-Glo(TM); Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo(TM); Kinase Assay result in less ambiguous data.