Promega

MLCK Kinase Enzyme System

Varenummer: V4496
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Recombinant human MLCK (1425-1776) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin-dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal. Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: MLCK, 10microg (Human, recombinant; amino acids 1425-1776). MW: ~70kDa. Substrate: MRCL3 Peptide (KKRPQRATSN-VFAM-NH2); derived from human myosin regulatory light chain MRCL3 (amino acid 11-24). Other: Reaction Buffer, DTT, Ca2+/Calmodulin Solution. MLCK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/91807/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.|
Profile More Compounds In-House: ADP-Glo(TM); Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo(TM); Kinase Assay result in less ambiguous data.

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Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at -70 C and the rest of the components at -20 C.|For Cat.# V4497: Patents Pending. U.S. Pat. No. 7,700,310 and other patents and patents pending. U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents. U.S. Pat. No. 7,741,067 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Kontaktperson(er) til dette produktet

Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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