Promega

PDGFR alpha Kinase Enzyme System

Varenummer: V3722
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Recombinant human PDGFR alpha (amino acids 550-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha (platelet-derived growth factor receptor alpha) is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity. Aberrant expression of PDGFR alpha has been linked to developmental abnormalities in vertebrate models and has been implicated in multiple disease states in humans. There is widespread expression of PDGFR alpha in renal cell types involved in fibrotic and sclerosing processes. PDGF and its receptor PDGFR alpha are inducers of fibrosis in the repair phase of inflammatory bowel disease, and they may also be involved in the active inflammatory phase. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: PDGFR alpha, 10ug (Human, recombinant; amino acids 550-end). MW: ~95kDa. Substrate: Poly (4:1 Glu, Tyr) Peptide. Other: Reaction Buffer, DTT. PDGFR alpha NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5156/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.|
Profile More Compounds In-House: ADP-Glo(TM); Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo(TM); Kinase Assay result in less ambiguous data.

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Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at -70°C and the rest of the components at -20°C.|For Cat.# V8011:Patents Pending.U.S. Pat. No. 7,700,310 and other patents and patents pending.U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending. U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.U.S. Pat. No. 7,741,067 and other patents and patents pending.The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Kontaktperson(er) til dette produktet

Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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