GoTaq G2 Polymerase is the second generation of GoTaq products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience. For superior convenience and improved yield, sensitivity and specificity, choose GoTaq G2 Hot Start Polymerase, which is bound to a proprietary antibody that blocks activity. Activity is restored during initial denaturation, allowing hot-start PCR. Available as a master mix or standalone enzyme. GoTaq G2 Hot Start Polymerase is supplied with 5X Green GoTaq Flexi Buffer, 5X Colorless GoTaq Flexi Buffer and 25mM MgCl2. The high-performance GoTaq G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94-95 degrees C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 degrees C for convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq G2 Hot Start Polymerase exhibits 5' --more than 3' exonuclease activity. The GoTaq G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template and go! The GoTaq G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. The GoTaq G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification.|
Robust reliable PCR for all your amplification needs.
Simplify reaction setup and save time with a ready-to-use master mix.
Prepare your reaction at room temperature, not on ice.
Eliminate nonspecific amplification with hot-start enzyme.
Use at no risk-backed by the Promega PCR Satisfaction Guarantee.