Promega

Kinase Selectivity Profiling System: TK-2, 8 x 50 reactions

Varenummer: V6852
Kort informasjon 8 x 50 reactions
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  • Spesifikasjoner
The Kinase Selectivity Profiling Systems are easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs organized in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions. Kinase Selectivity Profiling Systems offer kinases grouped either in single kinase family strips or as a general panel of kinases representative of the human kinome for a broad kinase profile. Each profiling system contains the reagents needed to complete a profile for a compound, including kinase reaction buffer, eight kinases in each multiwell strip and eight corresponding substrates and cofactors in another multiwell strip. The General Panel contains 24 kinases arranged in three 8-well strips. If you do not see a kinase selectivity profiling system that meets your experimental needs, you can design your own custom system using the custom kinase selectivity profiling system tool (www.promega.com/products/cell-signaling/kinase-assays/customize/).|

Fast Turnaround Time: Lead compounds can be profiled in-house in a matter of hours versus days when compounds are sent out.
Flexible Kinase Inhibitor Profiling: Each system has enough material to profile up to twenty compounds at a single dose or create a dose-response for two compounds against the eight kinases at once.
Fast and Simple Reaction Assembly: Two quick dilutions provide working stocks of kinases and substrate/cofactor solutions.
Optimized Kinase Activity for Inhibitor Profiling: All kinases have been optimized to provide 10-30% ADP production when assayed at 10µM ATP.
Formatted Strips Provide Access to Eight Kinases at One Time: Kinases from singular kinase families are grouped together for a more relevant selectivity profile.
Accurate: Accurately measure ADP levels at a wide range of starting ATP concentrations; activity measured truly reflects kinase activity and produces accurate IC50 values comparable to radioactivity-based assays.
Stable Luminescent Signal: Perform batch plate processing without need for strictly timed incubations; flexible.

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Ekstra spesifikasjoner
Store the Kinase Selectivity Profiling Systems below -65 C. Before use, thaw 5X Reaction Buffer A and 0.1M DTT at room temperature, and thaw the Substrate/Co-Factor Strip on ice. Immediately before use, thaw the Kinase Strip on ice, dilute and use immediately. After use, discard any remaining Kinase Working Stock and Substrate/Co-Factor Working Stock. Store any remaining 5X Reaction Buffer A and 0.1M DTT at -20 C for future use with the second Kinase Strip and Substrate/Co-Factor Strip.|

Kontaktperson(er) til dette produktet

Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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