The Kinase Selectivity Profiling Systems are easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs organized in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions. Kinase Selectivity Profiling Systems offer kinases grouped either in single kinase family strips or as a general panel of kinases representative of the human kinome for a broad kinase profile. Each profiling system contains the reagents needed to complete a profile for a compound, including kinase reaction buffer, eight kinases in each multiwell strip and eight corresponding substrates and cofactors in another multiwell strip. The General Panel contains 24 kinases arranged in three 8-well strips. If you do not see a kinase selectivity profiling system that meets your experimental needs, you can design your own custom system using the custom kinase selectivity profiling system tool (www.promega.com/products/cell-signaling/kinase-assays/customize/).|
Fast Turnaround Time: Lead compounds can be profiled in-house in a matter of hours versus days when compounds are sent out.
Flexible Kinase Inhibitor Profiling: Each system has enough material to profile up to twenty compounds at a single dose or create a dose-response for two compounds against the eight kinases at once.
Fast and Simple Reaction Assembly: Two quick dilutions provide working stocks of kinases and substrate/cofactor solutions.
Optimized Kinase Activity for Inhibitor Profiling: All kinases have been optimized to provide 10-30% ADP production when assayed at 10µM ATP.
Formatted Strips Provide Access to Eight Kinases at One Time: Kinases from singular kinase families are grouped together for a more relevant selectivity profile.
Accurate: Accurately measure ADP levels at a wide range of starting ATP concentrations; activity measured truly reflects kinase activity and produces accurate IC50 values comparable to radioactivity-based assays.
Stable Luminescent Signal: Perform batch plate processing without need for strictly timed incubations; flexible.