The PowerPlex 16 System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. The system allows the co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin): Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 loci is labeled with 6-carboxy-4',5'-dichloro-2',7'-dimethoxy-fluorescein (JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. The PowerPlex 16 System is compatible with the ABI PRISM 310, 3100 and 3100-Avant Genetic Analyzers, Applied Biosystems 3130 and 3130xl Genetic Analyzers and ABI PRISM 377 DNA Sequencer.|
Single Amplification: Amplify all 13 CODIS STR loci in one reaction.
Maximum Sensitivity: Analyze minute quantities of DNA. The system is optimized for use with 1ng of DNA; however, each lot performance tested to provide reproducible results with 0.5ng of DNA. Additional studies show interpretable results can be obtained with less than0.5ng of DNA.
Internal Lane Standard 600: This marker offers the greatest precision available for DNA typing. It is used in each gel lane or capillary injection to increase the precision of analysis.
Allelic Ladders: Allelic ladders for all 16 loci are provided to simplify interpretation.
Automatic Assignment of Genotypes: Panels and bins text files are required for use with GeneMapper(R), ID software and are available for download. The PowerTyper(TM); 16 Macro (available separately) facilitates data analysis, allowing automatic assignment of genotypes using the Genotyper(R), software. The PowerTyper(TM); Macros can be downloaded or ordered on CD-ROM.
Validation Studies: The PowerPlex(R), 16 System has undergone extensive validation efforts including internal validation, CODIS database validation and forensic casework validation.