Promega

PowerPlex 16 System 400 reactions

Varenummer: DC6530
Kort informasjon 400 reactions
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  • Spesifikasjoner
The PowerPlex 16 System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. The system allows the co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin): Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 loci is labeled with 6-carboxy-4',5'-dichloro-2',7'-dimethoxy-fluorescein (JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. The PowerPlex 16 System is compatible with the ABI PRISM 310, 3100 and 3100-Avant Genetic Analyzers, Applied Biosystems 3130 and 3130xl Genetic Analyzers and ABI PRISM 377 DNA Sequencer.|

Single Amplification: Amplify all 13 CODIS STR loci in one reaction.
Maximum Sensitivity: Analyze minute quantities of DNA. The system is optimized for use with 1ng of DNA; however, each lot performance tested to provide reproducible results with 0.5ng of DNA. Additional studies show interpretable results can be obtained with less than0.5ng of DNA.
Internal Lane Standard 600: This marker offers the greatest precision available for DNA typing. It is used in each gel lane or capillary injection to increase the precision of analysis.
Allelic Ladders: Allelic ladders for all 16 loci are provided to simplify interpretation.
Automatic Assignment of Genotypes: Panels and bins text files are required for use with GeneMapper(R), ID software and are available for download. The PowerTyper(TM); 16 Macro (available separately) facilitates data analysis, allowing automatic assignment of genotypes using the Genotyper(R), software. The PowerTyper(TM); Macros can be downloaded or ordered on CD-ROM.
Validation Studies: The PowerPlex(R), 16 System has undergone extensive validation efforts including internal validation, CODIS database validation and forensic casework validation.
Ekstra spesifikasjoner
Store at -20 C. The fluorescent primer pairs and allelic ladders are light-sensitive; therefore, minimize light exposure.
For product intended use please see Patents & Disclaimers tab.|U.S. Pat. Nos. 5,843,660, 6,479,235, 6,221,598 and 7,008,771, Australian Pat. No. 724531, Canadian Pat. Nos. 2,118,048 and 2,251,793 and other patents and patents pending. U.S. Pat. No. 6,238,863 and other patents and patents pending.STR loci are the subject of U.S. Pat. No. RE 37,984, German Pat. No. DE 38 34 636 C2 and other patents issued to the Max-Planck-Gesellschaft zur Förderung der Wissenschaften, e.V., Germany.The purchase of this product does not convey a license to use AmpliTaq Gold(R), DNA polymerase. You should purchase AmpliTaq Gold(R), DNA polymerase licensed for the forensic and human identity field directly from your authorized enzyme supplier.Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380, 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

Kontaktperson(er) til dette produktet

Monica Laukas 404 40 960 monica.laukas@nmas.no

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