Protein Kinase C is an 82kDa monomeric enzyme consisting of a C-terminal catalytic domain and a cysteine-rich N-terminal regulatory domain. The regulatory domain contains the sites for calcium and phospholipid binding and a pseudosubstrate subdomain, the target for PKC autophosphorylation. PKC is isolated from rat brain following the procedure of Walton and colleagues. The purified PKC consists primarily of alpha, beta and gamma isoforms with lesser amounts of delta and zeta isoforms.|
Highly Pure: PKC is greater than 90% pure as determined by SDS-PAGE.