The resin-based Wizard Plus Midipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. When using the standard protocol, the entire midiprep process can be completed in 90 minutes or less, yielding up to 200ug of high-quality DNA with no organic extractions or ethanol precipitations. Multiple midipreps can be easily processed at one time with the Vac-Man (20-sample capacity, Cat.# A7231) or Vac-Man Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard Midicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing or restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511). The system includes sufficient reagents for 25 DNA isolations from 10-100ml of liquid culture.|
Fast: Rapid batch column method used for DNA isolation.Safe: Eliminates the need for cesium chloride:ethidium bromide gradient centrifugation and does not require organic extractions.Reliable: Yields plasmid DNA of comparable quantity and quality to cesium chloride:ethidium bromide gradient techniques that are much more time- and labor-intensive.High Performance: DNA is suitable for restriction enzyme digestions, automated fluorescent DNA sequencing, transformation and subcloning.Confidence in Results: Purified DNA meets a target of more than98% accuracy over 500 bases using pGEM(R)-3Zf(+) Vector in BigDye(R) terminator sequencing.