Promega

Wizard Plus Megapreps DNA Purification System

Varenummer: A7300
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Wizard Plus Megapreps DNA Purification System provides a simple and rapid method for large-scale purifications of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high-copy-number plasmid DNA (200-20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511).|
Fast: Rapid batch binding and column washing method used for DNA isolationSafe: Eliminates the need for cesium chloride:ethidium bromide gradient centrifugation and does not require organic extractions.Reliable: Yields plasmid DNA of comparable quantity and quality to cesium chloride:ethidium bromide gradient techniques that are much more time- and labor-intensive.Yield: Each megaprep produces more than1mg of DNA from 1,000ml of bacterial culture when using a high-copy-number plasmid.Quality: DNA is suitable for restriction enzyme digestions, automated fluorescent DNA sequencing, transformation and subcloning.

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Store at 22-25°C.|U.S. Pat. Nos. 5,658,548 and 5,808,041 and other patents.

Kontaktperson(er) til dette produktet

Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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