The resin-based Wizard Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man (20-sample capacity, Cat.# A7231) or Vac-Man Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard Minicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor, such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511). The Wizard Minipreps DNA Purification Resin is used in the isolation and preparation of plasmid DNA in conjunction with the Wizard Plus Minipreps DNA Purification Systems. The resin is available with the systems and as a standalone product.|
High Performance: DNA is suitable for most molecular biology applications, including fluorescent sequencing.Confidence in Results: Purified DNA meets a target of more than98% accuracy over 500 bases using pGEM(R)-3Zf(+) Vector in BigDye(R) terminator sequencing.Fast: Entire procedure may be completed in 15 minutes or less.Convenient: No phenol extractions or ethanol precipitations required.