Set of dATP, dCTP, dGTP, dUTP

Varenummer: U1245
Kort informasjon 40 µmol each Highly pure (=99% triphosphate). Each dNTP supplied at 100mM in water for ease-of-use. Incorporate dUTP into PCR products and treat with uracil-N-glycosylase to inhibit subsequent amplifications
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High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dUTP (2'-Deoxyuridine, 5'-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is function-tested to ensure specific DNA amplification and the absence of nuclease activity.|
Dependable: PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzymes.Consistent: dUTP is ≥99% triphosphate, allowing highly consistent results.Convenient: Supplied at a convenient concentration (100mM in water) for ease-of-use in PCR and other applications.

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Ekstra spesifikasjoner
Store at -20°C. Avoid exposure to frequent temperature changes.|

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Claudia Emmanuel 951 51 950
Monica Laukas 404 40 960

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