The pALTER-MAX Vector is a 5,534bp plasmid. It contains the human cytomegalovirus (CMV) immediate-early enhancer/promoter region for strong, constitutive expression of cloned DNA inserts in a variety of mammalian cell types. The pALTER-MAX Vector as supplied is chloramphenicol-resistant and ampicillin-sensitive.|
High Expression: Protein expression driven by the human cytomegalovirus (CMV) immediate-early enhancer/promoter region allows high constitutive expression in a variety of cell lines. A chimeric intron composed of the 5´-splice site from the β-globin intron and the 3´-splice site from an IgG intron allows increased expression levels.Efficient: SV40 late polyadenylation signal for efficient RNA processing. T7 and T3 RNA polymerase promoters flank the multiple cloning site and allow RNA in vitro synthesis from sense or antisense strands.Flexible: Restriction sites flanking the CMV enhancer (BglII and SgfI) and the CMV promoter (SgfI and I-PpoI) allow for easy replacement of these regions by alternative regulatory regions.