The HaloTag Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag fusion proteins from mammalian cell culture lysates. HaloTag is a protein fusion tag engineered to form a highly specific and covalent bond with the HaloLink Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag Mammalian Protein Detection and Purification System (Cat.# G6795) also includes HaloTag TMRDirect Ligand. The simple-to-use fluorescent detection of the HaloTag fusion facilitates rapid optimization of expression and purification conditions.|
Purify More Protein: HaloLink™ Resin covalently binds more than7mg/ml of HaloTag(R) fusion protein (10X more capacity compared to FLAG(R)). Recovery is highly efficient, commonly more than75%.Higher Purity: Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (less than0.1%) nonspecific binding and a highly pure protein.Easily Scalable: Scale up and down, important for obtaining mg-plus quantities.Optimized for Mammalian Protein Expression: The HaloTag(R) platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct.
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Store Spin Columns at room temperature. Store HaloLink(TM); Resin at 4 C. Store HaloTEV Protease below -65 C. Store HaloTag(R), TMRDirect(TM); Ligand and powdered Protease Inhibitor Cocktail at -30 to -10 C. Reconstituted Protease Inhibitor Cocktail can be stored at 2-10 C for 12 months.|BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.Researchers may only use this product in their own research and may modify or link Promega HaloTag(R) ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
- HaloTag Mammalian Protein Detection and Purification System