ImProm-II Reverse Transcription System

Varenummer: A3800
Kort informasjon 100 reactions
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The ImProm-II Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II Reverse Transcriptase provided in the ImProm-II System together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1-20ul) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.|

Amenable to Full-Length RT-PCR: Reverse transcribe long RNA templates up to 8.9kb.
Microarray-Compatible: May be used to incorporate regular, Cy(R),3-modified, Cy(R),5-modified and amino-allyl-modified nucleotides.
Easy to Use: System provides all reagents necessary for efficient reverse transcription.
Scalable and Flexible: 1-20 ml of the initial reverse transcription reaction may be used in subsequent PCR, and the optimized buffer allows coupled RT-PCR.
RT Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25 C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
Versatile: Use with your thermostable DNA polymerase of choice.
Choose Your Configuration: Learn more about our custom options for this product at:

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Ekstra spesifikasjoner
Store at -20°C. Store Positive Control RNA at -70°C.|U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. No. 5,552,302.

Kontaktperson(er) til dette produktet

Claudia Emmanuel 951 51 950
Monica Laukas 404 40 960

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