The pSP64 Poly(A) Vector can be used as a standard cloning vector and for in vitro transcription from the SP6 promoter. The pSP64 Poly(A) Vector also can be used to generate poly(A)+ transcripts in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites. Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI (HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that contain a synthetic 3' poly(A) tail of 30 residues.|
In Vitro Transcription: The SP6 promoter is next to the polylinker.Generates Poly(A)+ Transcripts In Vitro: A stretch of 30 dA:dT residues are inserted between the SacI and EcoRI sites in the polylinker. Poly(A) tails can stabilize RNAs and lead to greater yields for in vitro translation reactions. Convenient: Multiple cloning region provides a selection of restriction sites for cloning.