SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only SP6 DNA or DNA cloned downstream from an SP6 promoter can serve as a template for SP6 RNA Polymerase-directed RNA synthesis.|
Specific: SP6 RNA Polymerase exhibits extremely high affinity and specificity for SP6 promoter sequences.
Highly Pure: SP6 RNA Polymerase is more than90% pure as determined by SDS polyacrylamide gel electrophoresis. Free of detectable levels of contaminating RNase and DNase activity (less than1% release).
Flexible: Will incorporate 32P, 33P, 3H and 35S nucleoside triphosphates.
Provided with 5X Reaction Buffer: Provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25 C), 30mM MgCl2, 10mM spermidine, 50mM NaCl.
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