pFN6A (HQ) Flexi Vector

Varenummer: C8511
Kort informasjon 20 µg
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The vectors below are used for inducible expression of HQ- and GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The HQ tag and polyhistidine tag (His) are comparable in their affinity for Ni ions and will bind to all His-binding surfaces and resins. In certain cases the HQ-tagged proteins can be eluted from the affinity columns at lower concentrations of imidazole—a property useful for some downstream applications such as enzymatic reactions. As with His tag, proteins can be expressed from bacterial, insect and mammalian systems and purified under either native or denaturing conditions. The GST tag has been successfully used to boost tagged protein solubility during E. coli expression. The Flexi Vector System is a simple, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector. pFN2A/K (GST) Flexi Vectors are designed for protein expression with an N-terminal GST tag in E. coli and T7 cell-free expression systems. pFN6A/K (HQ) Flexi Vectors are designed for protein expression with an N-terminal HQ tag in E. coli and T7 cell-free expression systems. pFC7A/K (HQ) Flexi Vectors are designed for protein expression with an C-terminal HQ in E. coli and T7 cell-free expression systems. pF1A/K T7 Flexi Vectors (Cat.# C8441, C8451) are designed for inducible expression of native untagged protein. Note: Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.|
Easy to Implement and Reliable: Choose between traditional His-affinity and GST-affinity resins for standard protein purification and prokaryotic expression applications. Cost-Effective: Technology for reusable and cost-efficient Ni (His-affinity) and gluthathione (GST-affinity) resins. Versatile Cloning: Choose from a variety of expression systems and fusion tag orientations and then transfer to others as required (for Flexi(R), system). Time Savings: Barnase insert (Flexi(R), system) decreases the number of background colonies, allowing efficient transfer of genetic constructs.

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Store vectors at -20 C.|All products on this page: Patent Pending.All products on this page: For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.All products on this page: European Pat. No. 1685247 and other patents pending.Cat.# C8461, C8471: This product or portions thereof is manufactured under license from Amrad Corporation Limited. For non-commercial research use only. All other uses require a license from Amrad Corporation Limited, Unit 6, 663 Victoria Street, Abbotsford, Victoria 3067, Australia, under U.S. Pat. No. 5,654,176, Australian Pat. No. 607511, Canadian Pat. No. 1338903 and other issued patents.All products on this page: Usage restrictions apply to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof.Usage Restrictions for the T7 Expression SystemThe T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of patents assigned to Brookhaven Science Associates, LLC (BSA). This technology, including bacteria, phage and plasmids that carry the gene for T7 RNA polymerase, is to be used for academic or nonprofit laboratory or licensed commercial research purposes only. By accepting or using the T7 expression technology you agree to be bound by the following conditions set forth by BSA. The initial purchaser may refuse to accept the conditions of this notice by returning this product and the enclosed materials to Promega unused. Academic and NonProfit LaboratoriesNo materials that contain the cloned gene for T7 RNA polymerase may be distributed further to third parties outside of your laboratory unless the recipient receives a copy of this assurance notice and agrees to be bound by its terms. This limitation applies to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof. Commercial LaboratoriesA license is required for any commercial use of the T7 expression system, including use of the T7 system for research purposes or for production purposes by any commercial entity. Information about commercial licenses may be obtained from the Licensing Office, Brookhaven National Laboratory, Upton, NY 11973, Telephone: 631-344-7134, FAX: 631-344-3729.

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Claudia Emmanuel 951 51 950
Monica Laukas 404 40 960

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