Promega

Bacterial Strain LE392, Glycerol Stock

Varenummer: K9981
Kort informasjon 500 µL
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Bacterial Strain LE392 is used for both genomic and cDNA cloning. LE392 lacks the E. coli K restriction system but is rec+. It is apermissive host, allowing both recombinant and parental phage to grow. Unlike Y1090, LE392 does not allow color selection of recombinants or IPTG induction of expression. Also, since LE392 contains lon protease activity, fusion proteins are often less stable in this host than in Y1090. When using this strain, phage titers tend to be higher than with Y1090 host cells. Therefore, we recommend (if color selection is not required) that LE392 be the primary strain for amplification of recombinant phage and for screening a cDNA library with nucleic acid probes. Genotype: hsdR514(r(k)-, m(k)+), glnV(supE44), tryT (supF58), lacY1 or Delta(lacIZY)6, galK2, galT22, metB1, trpR55.|

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Store at -70°C.|

Kontaktperson(er) til dette produktet

Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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